The United States has evacuated more than 300 citizens out of Wuhan.
The two flights left the Chinese city on Tuesday after the passengers were screened.
In a statement, the US State Department said the passengers will be subject to "Centres for Disease Control (CDC) screening, health observation, and monitoring requirements" upon arrival at one of two California military bases.
Authorities added that more flights for US citizens in Wuhan could be scheduled for Thursday.
It comes as China criticised the US of spreading fear over the coronavirus outbreak.
Hong Kong fire kills 55, hundreds missing as police blame 'gross negligence'
Pope Leo arrives in Turkey on closely watched first overseas trip
Train hits, kills 11 in China's worst rail accident in over a decade
US stops processing Afghan immigration requests after attack near White House
Indonesia races to evacuate stranded residents as flood death toll hits 28
